| Field | Specification |
|---|---|
| Target | |
| Alternative names | Paxillin;PXN; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Paxillin |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HEG-16 is a rabbit monoclonal antibody that recognizes paxillin (gene PXN) phosphorylated at Tyr118 in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 64.5 kDa, and the supplier reports an observed band at about 68 kDa.
Paxillin (PXN) is a focal adhesion adaptor protein that links integrin-based adhesions to the actin cytoskeleton. It is phosphorylated by FAK and Src and serves as a docking site for many signaling and structural proteins, so it is widely used as a marker in studies of cell adhesion and migration.
| Target | Paxillin (gene PXN; UniProt P49023, human) — phosphorylated at Tyr118 |
|---|---|
| Clone | HEG-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 64.5 kDa |
| Observed MW | 68 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human paxillin.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places paxillin in the cytoplasm and cell junction. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jing Liu et al. (2024) Proteomic analysis reveals the mechanism that low molecular weight hyaluronic acid enhances cell migration in keratinocyte. Journal of Pharmaceutical and Biomedical Analysis. 10.1016/j.jpba.2024.116402
