| Field | Specification |
|---|---|
| Target | |
| Alternative names | 6-pyruvoyl tetrahydrobiopterin synthase; PTP synthase; PTPS; PTS |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-PTS (S19) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 28P67 is a rabbit monoclonal antibody that recognizes 6-pyruvoyl tetrahydrobiopterin synthase (gene PTS) phosphorylated at Ser19 in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 16.4 kDa, and the supplier reports an observed band at about 18 kDa.
6-Pyruvoyl tetrahydropterin synthase (PTS) catalyzes the second step in de novo synthesis of tetrahydrobiopterin (BH4), the cofactor of phenylalanine, tyrosine and tryptophan hydroxylases and nitric oxide synthases. Loss of PTS activity is a known cause of BH4-deficient hyperphenylalaninemia.
| Target | 6-pyruvoyl tetrahydrobiopterin synthase (gene PTS; UniProt Q03393, human) — phosphorylated at Ser19 |
|---|---|
| Clone | 28P67 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 16.4 kDa |
| Observed MW | 18 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): HUVEC cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic phosphopeptide around Ser19 of human PTS.
Reactivity Notes
The supplier lists reactivity with human. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
