| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cell cycle checkpoint protein RAD17; hRad17; RF-C/activator 1 homolog; RAD17; R24L |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-Rad17 (S656) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 28R30 is a rabbit monoclonal antibody that recognizes cell cycle checkpoint protein RAD17 phosphorylated at Ser656 in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 77.1 kDa, and the supplier reports an observed band at about 85 kDa.
RAD17 is a checkpoint protein that forms an RFC-like clamp loader with RFC2–5. It loads the 9-1-1 (RAD9–RAD1–HUS1) clamp onto damaged DNA, enabling ATR-dependent CHK1 activation, and it is needed for genome stability and sustained cell growth.
| Target | Cell cycle checkpoint protein RAD17 (gene RAD17; UniProt O75943, human) — phosphorylated at Ser656 |
|---|---|
| Clone | 28R30 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 77.1 kDa |
| Observed MW | 85 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic phosphopeptide around Ser656 of human rad17.
Reactivity Notes
The supplier lists reactivity with human. UniProt places cell cycle checkpoint protein RAD17 in the nucleus and chromosome. Tissue expression noted by UniProt (human): Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
