| Field | Specification |
|---|---|
| Target | |
| Alternative names | RAF proto-oncogene serine/threonine-protein kinase;2.7.11.1;Proto-oncogene c-RAF;cRaf;Raf-1;RAF1;RAF; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-Raf1 (S43) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ICO-18 is a rabbit monoclonal antibody that recognizes RAF proto-oncogene serine/threonine-protein kinase (gene RAF1) phosphorylated at Ser43 in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 73.1 kDa, and the supplier reports an observed band at about 73 kDa.
RAF1 (c-Raf) is a serine/threonine kinase that passes signals from active Ras to MEK1/2 and then ERK1/2, a cascade that shapes proliferation, differentiation and survival. Its activity is controlled by phosphorylation at several regulatory residues, and it is studied widely in growth-factor signaling and in Ras-driven cancers.
| Target | RAF proto-oncogene serine/threonine-protein kinase (gene RAF1; UniProt P04049, human) — phosphorylated at Ser43 |
|---|---|
| Clone | ICO-18 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 73.1 kDa |
| Observed MW | 73 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human HeLa cells, rat PC-12 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic phosphopeptide around Ser43 of human raf1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places RAF proto-oncogene serine/threonine-protein kinase in the cytoplasm, cell membrane, mitochondrion and nucleus. Tissue expression noted by UniProt (human): In skeletal muscle, isoform 1 is more abundant than isoform 2. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
