| Field | Specification |
|---|---|
| Target | |
| Alternative names | Retinoblastoma-associated protein;p105-Rb;pRb;Rb;pp110;RB1; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Retinoblastoma |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BHC-18 is a rabbit monoclonal antibody that recognizes retinoblastoma-associated protein (gene RB1) phosphorylated at Ser780 in human samples and is validated for Western blot and IHC. The predicted molecular weight is 106.2 kDa, and the supplier reports an observed band at about 110 kDa.
The retinoblastoma protein (pRb, RB1) is a classic tumor suppressor that controls entry into S phase. In its underphosphorylated form it binds E2F transcription factors and recruits chromatin-modifying enzymes to silence cell cycle genes; phosphorylation by cyclin–CDK complexes releases E2F and allows DNA replication to begin. Loss of RB1 causes retinoblastoma, and pRb status is a standard readout in cell cycle and cancer research.
| Target | Retinoblastoma-associated protein (gene RB1; UniProt P06400, human) — phosphorylated at Ser780 |
|---|---|
| Clone | BHC-18 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 106.2 kDa |
| Observed MW | 110 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human retinoblastoma.
Reactivity Notes
The supplier lists reactivity with human. UniProt places retinoblastoma-associated protein in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Expressed in the retina. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
