| Field | Specification |
|---|---|
| Target | |
| Alternative names | Receptor-interacting serine/threonine-protein kinase 3; RIP-like protein kinase 3; Receptor-interacting protein 3; RIP-3; RIPK3; RIP3 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-RIP3 (S232) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 31R36 is a rabbit monoclonal antibody that recognizes receptor-interacting serine/threonine-protein kinase 3 (gene RIPK3) phosphorylated at Ser232 in mouse and rat samples and is validated for Western blot. The predicted molecular weight is 56.9 kDa, and the supplier reports an observed band at about 50 kDa.
RIPK3 is a serine/threonine kinase that drives necroptosis, a regulated form of necrotic cell death. Activated through RIPK1, ZBP1 or TRIF, it forms the necrosome and phosphorylates the pseudokinase MLKL, which then damages the plasma membrane. RIPK3 is studied in inflammation, host defense against viruses and tissue injury.
| Target | Receptor-interacting serine/threonine-protein kinase 3 (gene RIPK3; UniProt Q9Y572, human) — phosphorylated at Ser232 |
|---|---|
| Clone | 31R36 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 56.9 kDa |
| Observed MW | 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): rat spleen tissue, rat liver tissue, rat heart tissue, rat L6 cells, mouse spleen tissue, mouse liver tissue, mouse heart tissue, mouse L929 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic phosphopeptide around Ser232 of human RIP3.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places receptor-interacting serine/threonine-protein kinase 3 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Highly expressed in the pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
