| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Mothers against decapentaplegic homolog 5;MAD homolog 5;Mothers against DPP homolog 5;JV5-1;SMAD family member 5;SMAD 5;Smad5;hSmad5;SMAD5;MADH5; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human Phospho-Smad1/5/9 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Target | |
| UniProt # |
Overview
Anti-Phospho-Smad1/5/9 (S463/S465/S467) Rabbit Monoclonal Antibody is an antibody targeting SMAD5. Common applications include WB. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: HAF-19; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 130 kDa; calculated MW: 52258 MW.
Boster Bio Anti-Phospho-Smad1/5/9 (S463/S465/S467) Rabbit Monoclonal Antibody catalog # P01423. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: SMAD5 — Mothers against decapentaplegic homolog 5
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: HAF-19; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 130 kDa; Calculated: 52258 MW
- Phospho site(s): S463,S465
Biological background
Protein function (datasheet): Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
Cellular localization (datasheet): Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
Tissue details (datasheet): Ubiquitous.
Research relevance and current trends
- Commonly studied in contexts related to Developmental Biology,Epigenetics and Nuclear Signaling,Nuclear Signaling,Nuclear Signaling Pathways,Organogenesis,Signal Transduction,Signaling Pathway,Stem Cells,TGF Beta.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
