| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mothers against decapentaplegic homolog 2;MAD homolog 2;Mothers against DPP homolog 2;JV18-1;Mad-related protein 2;hMAD-2;SMAD family member 2;SMAD 2;Smad2;hSMAD2;SMAD2;MADH2, MADR2; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Smad2 |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HBC-19 is a rabbit monoclonal antibody that recognizes SMAD family member 2 (gene SMAD2) phosphorylated at Ser250 in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 52.3 kDa, and the supplier reports an observed band at about 58 kDa.
SMAD2 is a receptor-regulated SMAD that carries signals from TGF-β and activin receptors. After phosphorylation it forms a complex with SMAD4 that enters the nucleus to control gene transcription, so phospho-SMAD2 is a standard readout of TGF-β pathway activity in development, fibrosis and cancer.
| Target | SMAD family member 2 (gene SMAD2; UniProt Q15796, human) — phosphorylated at Ser250 |
|---|---|
| Clone | HBC-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 52.3 kDa |
| Observed MW | 58 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human HeLa cells, human HepG2 cells, human HT-1080 cells, rat heart tissue, rat skeletal muscle tissue, mouse heart tissue, mouse skeletal muscle tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:1000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human smad2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places SMAD family member 2 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Hangjie Fu et al. (2025) Ellagic acid alleviates iron overload-induced liver damage by mitigating ferroptosis through modulation of the TGFβ/Smad signaling pathway. Food Research International. 10.1016/j.foodres.2025.116590
Zhan Yuan et al. (2022) Human epididymis protein 4 aggravates airway inflammation and remodeling in chronic obstructive pulmonary disease. Respiratory Research. 10.1186/s12931-022-02040-7
Huang Bangrui et al. (2024) Scutellarin ameliorates diabetic nephropathy via TGF-β1 signaling pathway. Natural Products and Bioprospecting. 10.1007/s13659-024-00446-y
Yunxia Wang et al. (2024) CXCL3/TGF-β-Mediated Crosstalk Between CAFs and Tumor Cells Augments RCC Progression and Sunitinib Resistance. iScience. 10.1016/j.isci.2024.110224
Depeng Fang et al. (2019) Platelet-rich plasma promotes the regeneration of cartilage engineered by mesenchymal stem cells and collagen hydrogel via the TGF-β/SMAD signaling pathway. Journal of Cellular Physiology. 10.1002/jcp.28211
Juanping Zhao et al. (2024) Riboflavin protects against pancreatic cancer metastasis by targeting TGF-β receptor 1. Bioorganic Chemistry. 10.1016/j.bioorg.2024.107274
Jianhui Chen et al. (2018) Polysaccharides from Dendrobium officinale inhibit bleomycin-induced pulmonary fibrosis via the TGFβ1-Smad2/3 axis. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2018.07.056
Yong Tang et al. (2019) MDM2 promotes epithelial–mesenchymal transition through activation of Smad2/3 signaling pathway in lung adenocarcinoma. OncoTargets and Therapy. 10.2147/OTT.S185076
Hong Wei Yu et al. (2023) TSLP Induces Epithelial–Mesenchymal Transition in Nasal Epithelial Cells From Allergic Rhinitis Patients Through TGF-β1/Smad2/3 Signaling. American Journal of Rhinology & Allergy. 10.1177/19458924231193154
