| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mothers against decapentaplegic homolog 3;MAD homolog 3;Mad3;Mothers against DPP homolog 3;hMAD-3;JV15-2;SMAD family member 3;SMAD 3;Smad3;hSMAD3;SMAD3;MADH3; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-Smad3 (S423 + S425) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AHO-19 is a rabbit monoclonal antibody that recognizes SMAD family member 3 (gene SMAD3) phosphorylated at Ser423 in human and mouse samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 48.1 kDa, and the supplier reports an observed band at about 55 kDa.
SMAD3 is a receptor-regulated SMAD activated by TGF-β and activin receptors. Once phosphorylated it partners with SMAD4, and sometimes AP-1 factors, to control transcription of target genes, and it influences wound healing, bone and cartilage formation and fibrosis.
| Target | SMAD family member 3 (gene SMAD3; UniProt P84022, human) — phosphorylated at Ser423 |
|---|---|
| Clone | AHO-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 48.1 kDa |
| Observed MW | 55 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with confirmed staining (IHC): human gastric adenocarcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): A549 cells.
Immunogen
Synthetic phosphopeptide around Ser423 of human smad3 +.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places SMAD family member 3 in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Qi Wang et al. (2021) Local administration of liposomal-based Srpx2 gene therapy reverses pulmonary fibrosis by blockading fibroblast-to-myofibroblast transition. Theranostics. 10.7150/thno.61085
Hong Lei et al. (2020) Semaphorin 7A promotes endothelial to mesenchymal transition through ATF3 mediated TGF-β2/Smad signaling. Cell Death & Disease. 10.1038/s41419-020-02818-x
Yuying Yao et al. (2019) Activation of Slit2/Robo1 Signaling Promotes Tumor Metastasis in Colorectal Carcinoma through Activation of the TGF-β/Smads Pathway. Cells. 10.3390/cells8060635
Kye-Im Jeon et al. (2022) Defining the Role of Mitochondrial Fission in Corneal Myofibroblast Differentiation. Investigative Ophthalmology & Visual Science. 10.1167/iovs.63.4.2
Juanping Zhao et al. (2024) Riboflavin protects against pancreatic cancer metastasis by targeting TGF-β receptor 1. Bioorganic Chemistry. 10.1016/j.bioorg.2024.107274
Pengzhen Wang et al. (2021) Baicalin promotes chondrocyte viability and the synthesis of extracellular matrix through TGF-β/Smad3 pathway in chondrocytes. American Journal of Translational Research. PMID: 34650772
Fan Xiaorong et al. (2021) Differential expression of microRNAs in human endometrium after implantation of an intrauterine contraceptive device containing copper. Molecular Human Reproduction. 10.1093/molehr/gaab052
Lauren E. Hein et al. (2023) TGF-β1 activates neutrophil signaling and gene expression but not migration. PLoS One. 10.1371/journal.pone.0290886
Lei Hong et al. (2019) Reciprocal enhancement of thrombosis by endothelial-to-mesenchymal transition induced by iliac vein compression. Life Sciences. 10.1016/j.lfs.2019.116659
Fan Xiao-rong et al. (2025) The Regulatory Mechanism of MicroRNA-144-3p on Damage to Endometrial Epithelial Cells Exposed to Copper Ions In Vitro. Current Medical Science. 10.1007/s11596-025-00110-7
