| Field | Specification |
|---|---|
| Target | |
| Alternative names | Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src; SRC; SRC1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-Src (Y419) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone DDH-19 is a rabbit monoclonal antibody that recognizes proto-oncogene tyrosine-protein kinase Src phosphorylated at Tyr419 in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 59.8 kDa, and the supplier reports an observed band at about 60 kDa.
Src is a non-receptor tyrosine kinase that relays signals from integrins, growth factor receptors and other cell-surface receptors to pathways controlling adhesion, migration, proliferation and survival. Autophosphorylation at Tyr419 in the activation loop (Tyr416 in chicken numbering) marks the active kinase, so phospho-Tyr419 antibodies are used as a readout of Src activity.
| Target | Proto-oncogene tyrosine-protein kinase Src (gene SRC; UniProt P12931, human) — phosphorylated at Tyr419 |
|---|---|
| Clone | DDH-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 59.8 kDa |
| Observed MW | 60 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic phosphopeptide around Tyr419 of human src.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places proto-oncogene tyrosine-protein kinase Src in the cell membrane, mitochondrion inner membrane, nucleus and cytoplasm. Tissue expression noted by UniProt (human): Expressed ubiquitously. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ma Xiao-Lu et al. (2022) Identification of a novel Calpain-2-SRC feed-back loop as necessity for β-Catenin accumulation and signaling activation in hepatocellular carcinoma. Oncogene. 10.1038/s41388-022-02367-x
Jinsong Zhang et al. (2021) Increased secretion of VEGF-C from SiO2-induced pulmonary macrophages promotes lymphangiogenesis through the Src/eNOS pathway in silicosis. Ecotoxicology and Environmental Safety. 10.1016/j.ecoenv.2021.112257
Xiang Li et al. (2024) Quercetin improves the protection of hydroxysafflor yellow a against cerebral ischemic injury by modulating of blood-brain barrier and src-p-gp-mmp-9 signalling. Heliyon. 10.1016/j.heliyon.2024.e31002
