| Field | Specification |
|---|---|
| Target | |
| Alternative names | Signal transducer and activator of transcription 3;Acute-phase response factor;STAT3;APRF; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-STAT3 (S727) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
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Product Overview
Clone CAA-19 is a rabbit monoclonal antibody that recognizes signal transducer and activator of transcription 3 (gene STAT3) phosphorylated at Ser727 in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 88.1 kDa, and the supplier reports an observed band at about 88 kDa.
STAT3 is a transcription factor activated mainly by IL-6-family cytokines through gp130 and JAK kinases, and also by growth factors and leptin. Phosphorylated STAT3 dimerizes, enters the nucleus and regulates genes for acute-phase responses, cell survival and proliferation. It shapes Th17 differentiation and is persistently active in many cancers, making it a major research target.
| Target | Signal transducer and activator of transcription 3 (gene STAT3; UniProt P40763, human) — phosphorylated at Ser727 |
|---|---|
| Clone | CAA-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 88.1 kDa |
| Observed MW | 88 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic phosphopeptide around Ser727 of human STAT3.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places signal transducer and activator of transcription 3 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xing Zhang et al. (2023) Fu-Zheng-Tong-Luo formula promotes autophagy and alleviates idiopathic pulmonary fibrosis by controlling the Janus kinase 2/signal transducer and activator of transcription 3 pathway. Journal of Ethnopharmacology. 10.1016/j.jep.2023.116633
Yongjing Zhang et al. (2024) Corydalis yanhusuo extract and its pharmacological substances alleviate food allergy by inhibiting mast cells activation via PLC/PKC/STAT3 pathway. Journal of Ethnopharmacology. 10.1016/j.jep.2024.118809
Shaohua Cheng et al. (2022) TIAM2 promotes proliferation and invasion of osteosarcoma cells by activating the JAK2/STAT3 signaling pathway. Journal of Bone Oncology. 10.1016/j.jbo.2022.100461
Gui-yu Li et al. (2024) Curcumol inhibits hepatocellular carcinoma proliferation through miRNA-124/STAT3 pathway: Network pharmacology and experimental validation. Current Research in Biotechnology. 10.1016/j.crbiot.2024.100270
Xin-Liang Xu et al. (2023) Synthesis, biological activity and mechanism of action of novel allosecurinine derivatives as potential antitumor agents. Bioorganic & Medicinal Chemistry. 10.1016/j.bmc.2023.117234
Xin Zeng et al. (2024) α-linolenic acid ameliorates pentylenetetrazol-induced neuron apoptosis and neurological impairment in mice with seizures via down-regulating JAK2/STAT3 pathway. British Journal of Nutrition. 10.1017/S0007114524000989
Li Xin et al. (2013) Delivery of the co-expression plasmid pEndo-Si-Stat3 by attenuated Salmonella serovar typhimurium for prostate cancer treatment. Journal of Cancer Research and Clinical Oncology. 10.1007/s00432-013-1398-0
Wenyin Jin et al. (2019) Da-Cheng-Qi Decoction Alleviates Intestinal Injury in Rats with Severe Acute Pancreatitis by Inhibiting the JAK2-STAT3 Signaling Pathway. Evidence-based Complementary and Alternative Medicine. 10.1155/2019/3909468
Li‑Min Cui et al. (2014) Protein expression under sustained activation of signal transducer and activator of transcription‑3 in diethylnitrosamine‑induced rat liver carcinogenesis. Oncology Letters. 10.3892/ol.2014.2194
Xiaojie Liu et al. (2013) Puerarin partly counteracts the inflammatory response after cerebral ischemia/reperfusion via activating the cholinergic anti-inflammatory pathway. Neural Regeneration Research. 10.3969/j.issn.1673-5374.2013.34.004
