| Field | Specification |
|---|---|
| Target | |
| Alternative names | Signal transducer and activator of transcription 5A;STAT5A;STAT5; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-Stat5 (Y694) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EDD-19 is a rabbit monoclonal antibody that recognizes signal transducer and activator of transcription 5A (gene STAT5A) phosphorylated at Tyr694 in human and mouse samples and is validated for Western blot and IHC. The predicted molecular weight is 90.6 kDa, and the supplier reports an observed band at about 95 kDa.
STAT5A is a transcription factor activated by prolactin, growth hormone, IL-2, KIT ligand and other cytokines and growth factors. It binds GAS elements in target genes and is essential for prolactin-driven mammary gland development and milk protein expression, and it is also studied in hematopoiesis and lymphocyte signaling.
| Target | Signal transducer and activator of transcription 5A (gene STAT5A; UniProt P42229, human) — phosphorylated at Tyr694 |
|---|---|
| Clone | EDD-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 90.6 kDa |
| Observed MW | 95 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human thyroid tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic phosphopeptide around Tyr694 of human stat5.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places signal transducer and activator of transcription 5A in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Shi Yongjing et al. (2024) The E3 ubiquitin ligase FBXO38 maintains the antitumor function of natural killer cells by sustaining IL-15R signaling. Cancer Immunology Research. 10.1158/2326-6066.CIR-23-1061
Huichao Zhao et al. (2022) Folic acid intervention changes liver Foxp3 methylation and ameliorates the damage caused by Th17/Treg imbalance after long-term alcohol exposure. Food & Function. 10.1039/D1FO04267J
