| Field | Specification |
|---|---|
| Target | |
| Alternative names | Microtubule-associated protein tau;Neurofibrillary tangle protein;Paired helical filament-tau;PHF-tau;MAPT;MAPTL, MTBT1, TAU; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Tau |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 17M40 is a rabbit monoclonal antibody that recognizes microtubule-associated protein tau (gene MAPT) phosphorylated at Ser324 in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 78.9 kDa, and the supplier reports an observed band at about 79 kDa.
Tau (MAPT) is a microtubule-associated protein enriched in neuronal axons, where it binds and stabilizes microtubules and helps maintain axonal transport. Abnormal phosphorylation causes tau to detach from microtubules and form aggregates, the hallmark of neurofibrillary tangles in Alzheimer′s disease and other tauopathies, which makes it one of the most studied proteins in neurodegeneration research.
| Target | Microtubule-associated protein tau (gene MAPT; UniProt P10636, human) — phosphorylated at Ser324 |
|---|---|
| Clone | 17M40 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 78.9 kDa |
| Observed MW | 79 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): SH-SY5Y cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human tau.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places microtubule-associated protein tau in the cytoplasm, cell membrane and cell projection. Tissue expression noted by UniProt (human): Expressed in neurons. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
