| Field | Specification |
|---|---|
| Target | |
| Alternative names | Paired mesoderm homeobox protein 2B; Neuroblastoma Phox; NBPhox; PHOX2B homeodomain protein; Paired-like homeobox 2B; PHOX2B; PMX2B |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PHOX2B. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEFG-16 is a rabbit monoclonal antibody that recognizes paired mesoderm homeobox protein 2B (gene PHOX2B) in human and mouse samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 31.6 kDa, and the supplier reports an observed band at about 35 kDa.
PHOX2B is a paired-like homeodomain transcription factor that specifies noradrenergic and autonomic neuron identity, including the locus coeruleus and sympathetic ganglia. It helps switch on genes such as dopamine β-hydroxylase, and its germline mutations underlie congenital central hypoventilation syndrome and some familial neuroblastoma, making it a key marker in autonomic nervous system and neuroblastoma research.
| Target | Paired mesoderm homeobox protein 2B (gene PHOX2B; UniProt Q99453, human) |
|---|---|
| Clone | AEFG-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 31.6 kDa |
| Observed MW | 35 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human PHOX2B.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places paired mesoderm homeobox protein 2B in the nucleus. Tissue expression noted by UniProt (human): Expressed in neuroblastoma, brain and adrenal gland. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
