| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phytanoyl-CoA dioxygenase domain-containing protein 1; Protein PHYHD1; PHYHD1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PHYHD1 recombinant protein (Position: M1-Q250). Human PHYHD1 shares 87.2% and 88% amino acid (aa) sequence identity with mouse and rat PHYHD1, respectively. |
| Purification | |
| Reconstitution | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects phytanoyl-CoA dioxygenase domain-containing protein 1 (gene PHYHD1) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA.
PHYHD1 is an iron and 2-oxoglutarate-dependent dioxygenase that shares a domain with phytanoyl-CoA hydroxylase. Biochemical work shows that it can turn over 2-oxoglutarate to succinate but does not hydroxylate acyl-CoA substrates, so its physiological substrate and role remain unclear.
| Target | Phytanoyl-CoA dioxygenase domain-containing protein 1 (gene PHYHD1; UniProt Q5SRE7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
| Flow cytometry | Not specified by the supplier |
Immunogen
Recombinant human PHYHD1 fragment (Met1–Gln250), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 87.2% and 88%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
