Anti-PHYHIPL Antibody Picoband®

Overview

Anti-PHYHIPL Antibody Picoband® is an antibody targeting PHYHIPL. Common applications include WB, IHC, Flow Cytometry, ELISA. Key specifications include host: Rabbit; clonality: Polyclonal; isotype: IgG; reactivity: Human,Mouse,Rat; observed MW: 40 kDa; calculated MW: 42,40,6 kDa.

Boster Bio Anti-PHYHIPL Antibody Picoband® catalog # A14122-1. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Key elements and design rationale

• Target: PHYHIPL
• Antibody format: Host: Rabbit; Clonality: Polyclonal; Isotype: IgG
• Species reactivity: Human,Mouse,Rat
• Molecular weight guidance: Observed: 40 kDa; Calculated: 42,40,6 kDa

Biological background

Scientific background (datasheet): Phytanoyl-CoA hydroxylase-interacting protein-like is an enzyme that in humans is encoded by the PHYHIPL gene. PHYHIPL (phytanoyl-CoA 2-hydroxylase interacting protein-like), also known as phytanoyl-CoA hydroxylase-interacting protein-like, is a 376 amino acid protein that contains one fibronectin type-III domain and belongs to the PHYHIP family. Conserved in chimpanzee, canine, mouse, rat, chicken, zebrafish and Caenorhabditis elegans, PHYHIPL exists as three alternatively spliced isoforms. PHYHIPL is a down-regulated target of IRX1, a homeobox tumor suppressor gene linked to gastric carcinoma. PHYHIPL may also play a role in the development of the central system. The gene that encodes PHYHIPL maps to human chromosome 10q21.1.

Research relevance and current trends

• Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
• Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).

Common research applications

• Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
• ELISA: Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.
• Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
• Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.

Notes for experimental interpretation

• Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
• Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.

As a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.