| Field | Specification |
|---|---|
| Target | |
| Alternative names | Peptidase inhibitor 16; PI-16; Cysteine-rich secretory protein 9; CRISP-9; PSP94-binding protein; CD antigen CD364; PI16; CRISP9; PSPBP |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PI-16/PI16 recombinant protein (Position: L28-L363). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets peptidase inhibitor 16 (gene PI16) in human, mouse, rat and monkey samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 49.5 kDa, and the supplier reports an observed band at about 70–75 kDa.
PI16 (peptidase inhibitor 16) is a secreted protein of the CAP (cysteine-rich secretory protein) superfamily. It has been reported to limit cardiomyocyte growth, but its targets and physiological role are still being defined.
| Target | Peptidase inhibitor 16 (gene PI16; UniProt Q6UXB8, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat, Monkey |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 49.5 kDa |
| Observed MW | 70–75 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat, Monkey) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human, Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human Jurkat cells, human PC-3 cells, monkey heart tissue, rat heart tissue, rat testis tissue, mouse heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse intestine tissue, rat intestine tissue, human bladder cancer tissue, human pancreatic cancer tissue, human testicular cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): A431 cells, HEPA1–6 cells.
Immunogen
Recombinant human PI-16/PI16 fragment (Leu28–Leu363), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse, rat and monkey. UniProt describes peptidase inhibitor 16 as a secreted protein. Tissue expression noted by UniProt (human): Expressed in prostate, testis, ovary and intestine. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Suo Daqin et al. (2025) Fibroblast-derived PI16 enhances tumor immune-suppressive microenvironment via inducing Tregs differentiation. Cellular Oncology. 10.1007/s13402-025-01090-5
