| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha; PIK3R1; GRB1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PI 3 Kinase p85 alpha/PIK3R1 recombinant protein (Position: D117-Q153). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets phosphatidylinositol 3-kinase regulatory subunit alpha (gene PIK3R1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 83.6 kDa, and the supplier reports an observed band at about 85 kDa.
PIK3R1 encodes p85α and its shorter forms, the main regulatory subunits of class IA PI3K. They stabilize and hold the p110 catalytic subunit in check, and their SH2 domains bind phosphotyrosine on activated receptors to bring PI3K to the membrane, so PIK3R1 is central to insulin signaling, growth factor responses and cancer research.
| Target | Phosphatidylinositol 3-kinase regulatory subunit alpha (gene PIK3R1; UniProt P27986, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 83.6 kDa |
| Observed MW | 85 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Jurkat cells, human HeLa cells, rat PC-12 cells, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue, human lymphoma tissue, human testicular cancer tissue, mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): Caco-2 cells.
Samples with a confirmed signal (flow cytometry): HL-60 cells.
Immunogen
Recombinant human PI 3 Kinase p85 alpha/PIK3R1 fragment (Asp117–Gln153), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places phosphatidylinositol 3-kinase regulatory subunit alpha in the cytoplasm. Tissue expression noted by UniProt (human): Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhang Fang et al. (2026) Transcriptomics and metabolomics reveal functional nanoplastics-induced male reproductive damage and resveratrol antagonistic effects. Journal of Nanobiotechnology. 10.1186/s12951-026-04283-8
Xiang Luo et al. (2026) A Pathological Microenvironment–Responsive Catalytic Carbon Nanodots Release Platform Intercepting the ROS–Inflammation Cascade to Alleviate Intervertebral Disc Degeneration. Advanced Healthcare Materials. 10.1002/adhm.71151
Chaohui Wang et al. (2026) Rhodiosin promotes cerebral angiogenesis in mice with ischemic stroke via PI3K/Akt pathway activation. Phytomedicine. 10.1016/j.phymed.2026.158247
Yongya Wu et al. (2020) Effect and mechanism of PI3K/AKT/mTOR signaling pathway in the apoptosis of GC-1 cells induced by nickel nanoparticles. Chemosphere. 10.1016/j.chemosphere.2020.126913
Chen Guo et al. (2026) BEZ235 enhances the photodynamic antitumor efficacy of hypoxia-responsive BODIPY prodrug nanoparticles by inhibiting the PI3K/mTOR pathway and alleviating hypoxia. Colloids and Surfaces B-Biointerfaces. 10.1016/j.colsurfb.2026.115644
Zhiwang Wang et al. (2025) Therapeutic mechanism of Jinshui Liujunzi Decoction in treating COPD: targeting the PI3K/Akt signaling pathway. Journal of Ethnopharmacology. 10.1016/j.jep.2025.120070
Hong-Xin Cui et al. (2018) Hypoglycemic Mechanism of the Berberine Organic Acid Salt under the Synergistic Effect of Intestinal Flora and Oxidative Stress. Oxidative Medicine and Cellular Longevity. 10.1155/2018/8930374
Xiao-feng Wang et al. (2025) ApoE-T807-modified tanshinone IIA liposomes enhance cognitive function in APP/PS1 mice by inhibiting Tau phosphorylation via the PI3K/AKT signaling pathway. Journal of Drug Delivery Science and Technology. 10.1016/j.jddst.2025.107219
Chengjie Deng et al. (2024) Sappanone A attenuates rheumatoid arthritis via inhibiting PI3K/AKT/NF-κB and JAK2/STAT3 signaling pathways in vivo and in vitro. International Immunopharmacology. 10.1016/j.intimp.2024.113560
Qingyang Liu et al. (2024) Sinomenine Alleviates Rheumatoid Arthritis by Suppressing the PI3K-Akt Signaling Pathway, as Demonstrated Through Network Pharmacology, Molecular Docking, and Experimental Validation. Drug Design Development and Therapy. 10.2147/DDDT.S475959
