| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Copper chaperone for superoxide dismutase;Superoxide dismutase copper chaperone;CCS; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human PI 3 Kinase p85 alpha/PIK3R1 recombinant protein (Position: D117-Q153). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-PI 3 Kinase p85 alpha/PIK3R1 Antibody Picoband® is an antibody for PIK3R1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: PIK3R1 (Copper chaperone for superoxide dismutase); UniProt: P27986
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 85 kDa, calculated 29041 MW
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-PI 3 Kinase p85 alpha/PIK3R1 Antibody Picoband® catalog # A00318-1.
Biological background
Biological context: Delivers copper to copper zinc superoxide dismutase (SOD1).
Expression and localization notes: cellular localization: Cytoplasm ., tissue context: Ubiquitous..
Common research applications
- Western blotting (WB): Compare PIK3R1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of PIK3R1 in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify PIK3R1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Phosphatidylinositol 3-kinase regulatory subunit alpha is an enzyme that in humans is encoded by the PIK3R1 gene. Its gene is mapped to 5q13. the bovine PI3K p85 subunit consists of 2 closely related proteins, p85-alpha and p85-beta. They cloned cDNAs encoding both p85 subunits, each of which is a 724-amino acid polypeptide. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance.
- Cross reactivity: No cross-reactivity with other proteins
- Cellular localization: Cytoplasm .
- Tissue details: Ubiquitous.
- Research category: Cancer,Cardiovascular,Metabolism,Neuroscience,Neurotransmission,Plasma Membrane,Potassium Channels,Receptors / Channels,Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
