| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phosphatidylinositol 3-kinase regulatory subunit alpha;PI3-kinase regulatory subunit alpha;PI3K regulatory subunit alpha;PtdIns-3-kinase regulatory subunit alpha;Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha;PI3-kinase subunit p85-alpha;PtdIns-3-kinase regulatory subunit p85-alpha;PIK3R1;GRB1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human PI 3 Kinase p85 alpha, identical to the related rat and mouse sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects phosphatidylinositol 3-kinase regulatory subunit alpha (gene PIK3R1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 83.6 kDa, and the supplier reports an observed band at about 85 kDa.
PIK3R1 encodes p85α and its shorter forms, the main regulatory subunits of class IA PI3K. They stabilize and hold the p110 catalytic subunit in check, and their SH2 domains bind phosphotyrosine on activated receptors to bring PI3K to the membrane, so PIK3R1 is central to insulin signaling, growth factor responses and cancer research.
| Target | Phosphatidylinositol 3-kinase regulatory subunit alpha (gene PIK3R1; UniProt P27986, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 83.6 kDa |
| Observed MW | 85 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat, Mouse) |
|---|
Samples with a confirmed band (WB): MCF-7 cells, HeLa cells, COLO-320 cells, SW620 cells, rat brain tissue, rat lung tissue, rat PC-12 cells, mouse brain tissue, mouse HEPA1–6 cells, mouse RAW2467 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Synthetic peptide from the N-terminal region of human PI 3 Kinase p85 alpha. The peptide sequence is conserved in rat and mouse.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places phosphatidylinositol 3-kinase regulatory subunit alpha in the cytoplasm. Tissue expression noted by UniProt (human): Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xinming Su et al. (2016) Thymoquinone inhibits inflammation, neoangiogenesis and vascular remodeling in asthma mice. International Immunopharmacology. 10.1016/j.intimp.2016.05.018
Li Yin et al. (2017) Expressions Profiles of the Proteins Associated with Carbohydrate Metabolism in Rat Liver Regeneration. Biomed Research International. 10.1155/2017/8428926
Ren Kai et al. (2021) Preliminary Study on the Antibacterial and Cytotoxic Effects of the Synthetic New Peptide NJP9-A. International Journal of Peptide Research and Therapeutics. 10.1007/s10989-021-10246-4
