| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phosphatidylinositol 3-kinase regulatory subunit alpha;PI3-kinase regulatory subunit alpha;PI3K regulatory subunit alpha;PtdIns-3-kinase regulatory subunit alpha;Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha;PI3-kinase subunit p85-alpha;PtdIns-3-kinase regulatory subunit p85-alpha;PIK3R1;GRB1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PI 3 Kinase p85 alpha |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone CGB-16 is a rabbit monoclonal antibody that recognizes phosphatidylinositol 3-kinase regulatory subunit alpha (gene PIK3R1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 83.6 kDa, and the supplier reports an observed band at about 85 kDa.
PIK3R1 encodes p85α and its shorter forms, the main regulatory subunits of class IA PI3K. They stabilize and hold the p110 catalytic subunit in check, and their SH2 domains bind phosphotyrosine on activated receptors to bring PI3K to the membrane, so PIK3R1 is central to insulin signaling, growth factor responses and cancer research.
| Target | Phosphatidylinositol 3-kinase regulatory subunit alpha (gene PIK3R1; UniProt P27986, human) |
|---|---|
| Clone | CGB-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 83.6 kDa |
| Observed MW | 85 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Immunogen
Synthetic peptide based on the sequence of human PI 3 Kinase p85 alpha.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places phosphatidylinositol 3-kinase regulatory subunit alpha in the cytoplasm. Tissue expression noted by UniProt (human): Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Aslıhan ŞENGELEN et al. (2024) Rosmarinic acid attenuates glioblastoma cells and spheroids’ growth and EMT/stem-like state by PTEN/PI3K/AKT downregulation and ERK-induced apoptosis. Phytomedicine. 10.1016/j.phymed.2024.156060
İlker Güleç et al. (2021) The calcimimetic R-568 attenuates subarachnoid hemorrhage-induced vasospasm through PI3K/Akt/eNOS signaling pathway in the rat model. Brain Research. 10.1016/j.brainres.2021.147508
Erhamit Okutan et al. (2025) Aloperine treatment attenuates acute spinal cord injury by reducing oxidative, inflammatory, and apoptotic responses via PI3K/AKT/NF-κB signaling in a rat contusion model. Neuroscience Letters. 10.1016/j.neulet.2025.138203
