| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform;PI3-kinase subunit beta;PI3K-beta;PI3Kbeta;PtdIns-3-kinase subunit beta;2.7.1.153;Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta;PtdIns-3-kinase subunit p110-beta;p110beta;PIK3CB;PIK3C1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PI3 Kinase p110 beta |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 30P53 is a rabbit monoclonal antibody that recognizes phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform (gene PIK3CB) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 122.8 kDa, and the supplier reports an observed band at about 123 kDa.
PIK3CB encodes p110β, a class IA PI3K catalytic subunit that produces PIP3 and activates AKT signaling. Unlike p110α, it can be activated by G protein-coupled receptors as well as tyrosine kinases, and it is studied in platelet function, insulin signaling and PTEN-deficient tumors.
| Target | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform (gene PIK3CB; UniProt P42338, human) |
|---|---|
| Clone | 30P53 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 122.8 kDa |
| Observed MW | 123 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with confirmed staining (IHC): human colon tissue, human colon cancer tissue, mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human PI3 Kinase p110 beta.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed ubiquitously. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Na Yang et al. (2025) Bioactive compound combinations from Rhodiola tangutica alleviate pulmonary vascular remodeling in high-altitude pulmonary hypertension rats through the PI3K–AKT pathway. Frontiers in Pharmacology. 10.3389/fphar.2025.1582677
Bin Hou et al. (2025) Luteolin alleviates right ventricular hypertrophy in high altitude pulmonary hypertension rats by regulating PI3K/AKT/mTOR signalling pathway. European Journal of Pharmacology. 10.1016/j.ejphar.2025.177898
Hong-bing Wu et al. (2019) Avβ3 single-stranded DNA aptamer attenuates vascular restenosis via Ras-PI3K/MAPK pathway in rats after percutaneous transluminal coronary angioplasty. Artificial Organs. 10.1111/aor.13622
Xintang Zhan et al. (2019) Comparative profiling of chondrogenic differentiation of mesenchymal stem cells (MSCs) driven by two different growth factors. Cell Biochemistry and Function. 10.1002/cbf.3404
Ying Liu et al. (2015) Vibration exercise decreases insulin resistance and modulates the insulin signaling pathway in a type 2 diabetic rat model. International Journal of Clinical and Experimental Medicine. PMID: 26550236
