| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phosphatidylinositol 3-kinase regulatory subunit alpha;PI3-kinase regulatory subunit alpha;PI3K regulatory subunit alpha;PtdIns-3-kinase regulatory subunit alpha;Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha;PI3-kinase subunit p85-alpha;PtdIns-3-kinase regulatory subunit p85-alpha;PIK3R1;GRB1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PI3 Kinase p85 alpha |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 17P38 is a rabbit monoclonal antibody that recognizes phosphatidylinositol 3-kinase regulatory subunit alpha (gene PIK3R1) in human, mouse and rat samples and is validated for ICC/IF and Western blot. The predicted molecular weight is 83.6 kDa, and the supplier reports an observed band at about 84 kDa.
PIK3R1 encodes p85α and its shorter forms, the main regulatory subunits of class IA PI3K. They stabilize and hold the p110 catalytic subunit in check, and their SH2 domains bind phosphotyrosine on activated receptors to bring PI3K to the membrane, so PIK3R1 is central to insulin signaling, growth factor responses and cancer research.
| Target | Phosphatidylinositol 3-kinase regulatory subunit alpha (gene PIK3R1; UniProt P27986, human) |
|---|---|
| Clone | 17P38 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 83.6 kDa |
| Observed MW | 84 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | Not specified by the supplier |
Samples with a confirmed band (WB): human Jurkat cells, human K562 cells, human 293T cells, rat brain tissue, rat PC-12 cells, mouse brain tissue, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human PI3 Kinase p85 alpha.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places phosphatidylinositol 3-kinase regulatory subunit alpha in the cytoplasm. Tissue expression noted by UniProt (human): Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jia-ning Gong et al. (2026) Platycodin D alleviates idiopathic pulmonary fibrosis by targeting PPP2R1A and regulating the PP2A-mediated PI3K/Akt pathway to inhibit fibroblast transdifferentiation. Phytomedicine. 10.1016/j.phymed.2026.158000
Ning Zhang et al. (2026) Safflower washing medicine alleviating acute soft tissue injury via PI3K/Akt pathway. Journal of Ethnopharmacology. 10.1016/j.jep.2026.121239
Yang Fang et al. (2025) Smart biodegradable hydrogels incorporating a photoactive Ir(III) complex for NIR-triggered oral cancer treatment. Cancer Nanotechnology. 10.1186/s12645-025-00336-z
Shuli Man et al. (2020) Curcumin-enhanced antitumor effects of sorafenib via regulating the metabolism and tumor microenvironment. Food & Function. 10.1039/C9FO01901D
Liu Xiao et al. (2019) Magnolol Prevents Acute Alcoholic Liver Damage by Activating PI3K/Nrf2/PPARγ and Inhibiting NLRP3 Signaling Pathway. Frontiers in Pharmacology. 10.3389/fphar.2019.01459
Fan Yang et al. (2025) Mulberry leaf flavonoid extract inhibits high glucose-induced ferroptosis by regulating the PI-3K/Akt/Nrf2/GPX4 pathway in HT22 mouse hippocampal neuronal cells. Experimental Cell Research. 10.1016/j.yexcr.2025.114848
Jing Li et al. (2025) Preconditioning with acteoside ameliorates myocardial ischemia‑reperfusion injury by targeting HSP90AA1 and the PI3K/Akt signaling pathway. Molecular Medicine Reports. 10.3892/mmr.2025.13442
Zhihao Xu et al. (2025) Transcriptome analysis reveals that the injection of mesenchymal stem cells remodels extracellular matrix and complement components of the brain through PI3K/AKT/FOXO1 signaling pathway in a neuroinflammation mouse model. Genomics. 10.1016/j.ygeno.2025.111033
Fan Qiu et al. (2019) Mulberry Bark Alleviates Effect of STZ Inducing Diabetic Mice through Negatively Regulating FoxO1. Evidence-based Complementary and Alternative Medicine. 10.1155/2019/2182865
Shiyu Yan et al. (2025) The Combination of Phytic Acid and Inositol Alleviates Metastasis of Colorectal Cancer in Mice by Inhibiting PI3K/AKT Pathway and M2 Macrophage Polarization. Biological & Pharmaceutical Bulletin. 10.1248/bpb.b25-00188
