| Field | Specification |
|---|---|
| Target | |
| Alternative names | PRKCA-binding protein; Protein interacting with C kinase 1; Protein kinase C-alpha-binding protein; PICK1; PRKCABP |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PICK1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 19P66 is a rabbit monoclonal antibody that recognizes PRKCA-binding protein (gene PICK1) in human, mouse and rat samples and is validated for Western blot, immunoprecipitation and flow cytometry. The predicted molecular weight is 46.6 kDa, and the supplier reports an observed band at about 50 kDa.
PICK1 is a PDZ- and BAR-domain adapter protein that binds and organizes membrane proteins carrying PDZ-binding motifs, including PKCα and AMPA receptor GluA2 subunits. By controlling AMPA receptor trafficking and internalization at synapses, it influences synaptic plasticity, and it is also studied in sperm development and membrane trafficking.
| Target | PRKCA-binding protein (gene PICK1; UniProt Q9NRD5, human) |
|---|---|
| Clone | 19P66 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 46.6 kDa |
| Observed MW | 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human PICK1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places PRKCA-binding protein in the cytoplasm, membrane, postsynaptic density and synapse. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
