| Field | Specification |
|---|---|
| Target | |
| Alternative names | PTB-containing, cubilin and LRP1-interacting protein; P-CLI1; Phosphotyrosine interaction domain-containing protein 1; Protein NYGGF4; PID1; NYGGF4; PCLI1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PID1 recombinant protein (Position: M1-K227). Human PID1 shares 97.3% amino acid (aa) sequence identity with mouse PID1. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets PTB-containing, cubilin and LRP1-interacting protein (gene PID1) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA.
PID1 (also called NYGGF4) is a cytoplasmic protein with a phosphotyrosine-binding domain that interacts with LRP1. It has been linked to preadipocyte proliferation and insulin signaling in adipose tissue, though its mechanisms are still being clarified.
| Target | PTB-containing, cubilin and LRP1-interacting protein (gene PID1; UniProt Q7Z2X4, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human PID1 fragment (Met1–Lys227), expressed in E. coli. Sequence identity with the mouse ortholog: 97.3%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places PTB-containing, cubilin and LRP1-interacting protein in the cytoplasm. Tissue expression noted by UniProt (human): Expressed in subcutaneous fat, heart, skeletal muscle, brain, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocyte.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
