| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phosphoinositide 3-kinase adapter protein 1; B-cell adapter for phosphoinositide 3-kinase; B-cell phosphoinositide 3-kinase adapter protein 1; PIK3AP1; BCAP |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PIK3AP1 recombinant protein (Position: H78-R583). Human PIK3AP1 shares 83.8% amino acid (aa) sequence identity with mouse PIK3AP1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects phosphoinositide 3-kinase adapter protein 1 (gene PIK3AP1) in human samples and is validated for Western blot and ELISA. The predicted molecular weight is 90.4 kDa, and the supplier reports an observed band at about 90 kDa.
PIK3AP1 (also called BCAP) is a signaling adapter that couples the B-cell receptor, as well as Toll-like receptors and IL-1 family receptors, to PI3K activation. By offering a binding site for the p85 regulatory subunit of PI3K, it supports B-cell development and tempers inflammatory responses in innate immune cells.
| Target | Phosphoinositide 3-kinase adapter protein 1 (gene PIK3AP1; UniProt Q6ZUJ8, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 90.4 kDa |
| Observed MW | 90 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HEL cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human PIK3AP1 fragment (His78–Arg583), expressed in E. coli. Sequence identity with the mouse ortholog: 83.8%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places phosphoinositide 3-kinase adapter protein 1 in the cytoplasm and cell membrane. Tissue expression noted by UniProt (human): Expressed in natural killer (NK) cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
