Anti-PIM2 Rabbit Monoclonal Antibody

Overview

This product is an anti-PIM2 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 24P65; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, ICC, IF, IP (as provided in the source record). Boster Bio Anti-PIM2 Rabbit Monoclonal Antibody catalog # M03053-2. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

• Target: PIM2 (Serpin H1).
• Antibody format: Monoclonal; clone 24P65; isotype IgG.
• Host: Rabbit.
• Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

PIM2 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen. Reported cellular localization context: Endoplasmic reticulum lumen. Tissue expression notes (as provided): Detected in brain.

Research relevance and current trends

• Research context keywords from the source record include: Chaperones,Developmental Biology,Heat Shock Proteins,Organogenesis,Protein Trafficking,Signal Transduction,Skeletal Development.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
• Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.

Workflow ideas (metafield): Validate PIM2 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect PIM2 expression by Western blot in cell or tissue lysates, Localize PIM2 by immunofluorescence/immunocytochemistry in cultured cells, Enrich PIM2 by immunoprecipitation from lysates for downstream analysis

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 34-44 kDa; calculated MW: 46441 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 34-44 kDa
• Cellular localization (provided): Endoplasmic reticulum lumen.
• Tissue details (provided): Detected in brain.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.