| Field | Specification |
|---|---|
| Target | |
| Alternative names | Piwi-like protein 1; PIWIL1; HIWI |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PIWIL1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18P89 is a rabbit monoclonal antibody that recognizes piwi-like protein 1 (gene PIWIL1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 98.6 kDa, and the supplier reports an observed band at about 99 kDa.
PIWIL1 (HIWI) is a Piwi-family Argonaute protein that binds piRNAs in the germline. Guided by these small RNAs, it cleaves transposon transcripts and helps silence transposable elements during spermatogenesis, protecting genome integrity, and it is also studied for its aberrant expression in some tumors.
| Target | Piwi-like protein 1 (gene PIWIL1; UniProt Q96J94, human) |
|---|---|
| Clone | 18P89 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 98.6 kDa |
| Observed MW | 99 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human PIWIL1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places piwi-like protein 1 in the cytoplasm. Tissue expression noted by UniProt (human): Expressed in spermatocytes and spermatids. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
