| Field | Specification |
|---|---|
| Target | |
| Alternative names | cAMP-dependent protein kinase type II-beta regulatory subunit;PRKAR2B; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PKA 2 beta |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABBB-16 is a rabbit monoclonal antibody that recognizes cAMP-dependent protein kinase type II-beta regulatory subunit (gene PRKAR2B) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 46.3 kDa, and the supplier reports an observed band at about 46 kDa.
PKA RIIβ (PRKAR2B) is a type II regulatory subunit of cAMP-dependent protein kinase A, abundant in brain and adipose tissue. It restrains the catalytic subunits until cAMP rises and uses A-kinase anchoring proteins to tether PKA to defined subcellular sites, and it is studied in neuronal signaling and energy balance.
| Target | CAMP-dependent protein kinase type II-beta regulatory subunit (gene PRKAR2B; UniProt P31323, human) |
|---|---|
| Clone | ABBB-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 46.3 kDa |
| Observed MW | 46 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human fetal brain.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human PKA 2 beta.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cAMP-dependent protein kinase type II-beta regulatory subunit in the cytoplasm and cell membrane. Tissue expression noted by UniProt (human): Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Li Si-yuan et al. (2025) Buyang Huanwu Decoction Promotes Recovery after Spinal Cord Injury by Regulating cAMP/PKA/NF-κB p65 Pathway. Chinese Journal of Integrative Medicine. 10.1007/s11655-025-4201-6
