| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein kinase C alpha type; PKC-A; PKC-alpha; PRKCA; PKCA; PRKACA |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PKC Alpha/PRKCA recombinant protein (Position: Q142-V672). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets protein kinase C alpha type (gene PRKCA) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 76.8 kDa, and the supplier reports an observed band at about 80 kDa.
PKCα (PRKCA) is a conventional protein kinase C isoform activated by calcium, diacylglycerol and phospholipids. It phosphorylates targets such as RAF1 and BCL2 and feeds into ERK/MAPK signaling, influencing cell proliferation, survival, adhesion and migration, and it is studied in cancer, cardiac function and platelet biology.
| Target | Protein kinase C alpha type (gene PRKCA; UniProt P17252, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 76.8 kDa |
| Observed MW | 80 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1x1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Jurkat cells, human 293T cells, human HeLa cells, human K562 cells, rat C6 cells, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): SiHa cells.
Immunogen
Recombinant human PKC Alpha/PRKCA fragment (Gln142–Val672), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places protein kinase C alpha type in the cytoplasm, cell membrane, mitochondrion membrane and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ying Wei et al. (2026) Systematic toxicology reveals dioctyl terephthalate as a hazardous agent on autism spectrum disorder-like behaviors: Evidence from network toxicology and zebrafish models. Journal of Environmental Sciences. 10.1016/j.jes.2026.03.015
Kai Zhang et al. (2024) Omics-based pharmacological evaluation reveals Yuanhu Zhitong oral liquid ameliorates arthritis by regulating PKC/ERK/NF-κB signaling pathway. Journal of Ethnopharmacology. 10.1016/j.jep.2024.118289
