| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein kinase C eta type; PKC-L; nPKC-eta; PRKCH; PKCL; PRKCL |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PKC eta |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADAH-16 is a rabbit monoclonal antibody that recognizes protein kinase C eta type (gene PRKCH) in human, mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 77.8 kDa, and the supplier reports an observed band at about 78 kDa.
PKCη (PRKCH) is a calcium-independent, diacylglycerol-activated novel protein kinase C enriched in epithelial tissues. In keratinocytes it promotes growth arrest and differentiation, and it also helps maintain epithelial tight junctions by phosphorylating occludin, so it is studied in skin biology, barrier function and T-cell signaling.
| Target | Protein kinase C eta type (gene PRKCH; UniProt P24723, human) |
|---|---|
| Clone | ADAH-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 77.8 kDa |
| Observed MW | 78 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human PKC eta.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places protein kinase C eta type in the cytoplasm. Tissue expression noted by UniProt (human): Most abundant in lung, less in heart and skin.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
