| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein kinase C gamma type; PKC-gamma; PRKCG; PKCG |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PKC gamma |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 19P37 is a rabbit monoclonal antibody that recognizes protein kinase C gamma type (gene PRKCG) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 78.4 kDa, and the supplier reports an observed band at about 78 kDa.
PKCγ (PRKCG) is a conventional protein kinase C expressed mainly in neurons of the brain and spinal cord. It is activated by calcium and diacylglycerol and phosphorylates glutamate receptors and other synaptic proteins, linking it to synaptic plasticity and pain processing; mutations in PRKCG cause spinocerebellar ataxia type 14.
| Target | Protein kinase C gamma type (gene PRKCG; UniProt P05129, human) |
|---|---|
| Clone | 19P37 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 78.4 kDa |
| Observed MW | 78 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): rat brain tissue, rat cerebellum tissue, mouse brain tissue, mouse cerebellum tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse cerebellum tissue, rat cerebellum tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human PKC gamma.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places protein kinase C gamma type in the cytoplasm, cell membrane, synapse and cell projection. Tissue expression noted by UniProt (human): Expressed in Purkinje cells of the cerebellar cortex. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Na Li et al. (2008) Endothelin receptor antagonist CPU0213 and vitamin E reverse downregulation of FKBP12.6 and SERCA2a: A role of hyperphosphorylation of PKCε. European Journal of Pharmacology. 10.1016/j.ejphar.2008.06.080
Jing Liu et al. (2006) Catalpol increases hippocampal neuroplasticity and up-regulates PKC and BDNF in the aged rats. Brain Research. 10.1016/j.brainres.2006.09.058
