Anti-PKC theta Rabbit Monoclonal Antibody

Overview

This product is an anti-PRKCQ antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 18P99; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB (as provided in the source record). Boster Bio Anti-PKC theta Rabbit Monoclonal Antibody catalog # M01293. Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

• Target: PRKCQ (Paired box protein Pax-2).
• Antibody format: Monoclonal; clone 18P99; isotype IgG.
• Host: Rabbit.
• Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

PRKCQ (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Transcription factor that may have a role in kidney cell differentiation (PubMed:24676634). Has a critical role in the development of the urogenital tract, the eyes, and the CNS. . Reported cellular localization context: Nucleus. Tissue expression notes (as provided): Expressed in primitive cells of the kidney, ureter, eye, ear and central nervous system.

Research relevance and current trends

• Research context keywords from the source record include: Cell Type Marker,Developmental Biology,Developmental Families,Domain Families,Epigenetics and Nuclear Signaling,Neuroscience,Organogenesis,Transcription,Transcription Factors.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.

Workflow ideas (metafield): Validate PRKCQ antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect PRKCQ expression by Western blot in cell or tissue lysates, Compare relative PRKCQ levels across experimental conditions (dose/time-course) using antibody-based readouts

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 130 kDa; calculated MW: 44706 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 130 kDa
• Cellular localization (provided): Nucleus.
• Tissue details (provided): Expressed in primitive cells of the kidney, ureter, eye, ear and central nervous system.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.