| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phospholipase A2, membrane associated; GIIC sPLA2; Group IIA phospholipase A2; Non-pancreatic secretory phospholipase A2; NPS-PLA2; Phosphatidylcholine 2-acylhydrolase 2A; PLA2G2A; PLA2B; PLA2L; RASF-A |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PLA2G2A |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25P80 is a rabbit monoclonal antibody that recognizes phospholipase A2, membrane associated (gene PLA2G2A) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 16.1 kDa, and the supplier reports an observed band at about 16 kDa.
Group IIA secreted phospholipase A2 is a Ca2+-dependent enzyme that hydrolyzes anionic phospholipids, especially those in bacterial membranes. It is a strong antibacterial factor against Gram-positive bacteria and an acute-phase protein that rises during inflammation, and it is studied in host defense, inflammatory disease and intestinal tumorigenesis in mice.
| Target | Phospholipase A2, membrane associated (gene PLA2G2A; UniProt P14555, human) |
|---|---|
| Clone | 25P80 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 16.1 kDa |
| Observed MW | 16 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|
Samples with a confirmed band (WB): human A549 cells, human HepG2 cells, human RT4 cells, human Hacat cells, rat small intestine tissue, rat C6 cells, mouse small intestine tissue, mouse EL-4 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human PLA2G2A.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes phospholipase A2, membrane associated as secreted and also located in the cell membrane and mitochondrion outer membrane. Tissue expression noted by UniProt (human): Expressed in various tissues including heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, prostate, ovary, colon and small intestine. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Lingxin Zhuo et al. (2025) Qifu Decoction Alleviates Lipopolysaccharide-Induced Myocardial Dysfunction by Inhibiting TLR4/NF-κB/NLRP3 Inflammatory Pathway and Activating PPARα/CPT Pathway. Pharmaceuticals. 10.3390/ph18081109
Cuiping Long et al. (2024) Sini Decoction alleviates inflammation injury after myocardial infarction through regulating arachidonic acid metabolism. Chinese Herbal Medicines. 10.1016/j.chmed.2023.12.004
