| Field | Specification |
|---|---|
| Target | |
| Alternative names | Group 3 secretory phospholipase A2; Group III secretory phospholipase A2; GIII sPLA2; sPLA2-III; Phosphatidylcholine 2-acylhydrolase 3; PLA2G3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PLA2G3 recombinant protein (Position: E74-R358). Human PLA2G3 shares 66.8% amino acid (aa) sequence identity with mouse PLA2G3. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes group 3 secretory phospholipase A2 (gene PLA2G3) in human and mouse samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 57.2 kDa, and the supplier reports an observed band at about 65 kDa.
Group III secreted phospholipase A2 is a Ca2+-dependent enzyme related to bee venom PLA2. It hydrolyzes membrane and lipoprotein phospholipids and has been studied in lipid mediator production, sperm maturation, mast cell function and lipoprotein remodeling.
| Target | Group 3 secretory phospholipase A2 (gene PLA2G3; UniProt Q9NZ20, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 57.2 kDa |
| Observed MW | 65 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human K562 cells, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): HepG2 cells.
Immunogen
Recombinant human PLA2G3 fragment (Glu74–Arg358), expressed in E. coli. Sequence identity with the mouse ortholog: 66.8%.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt describes group 3 secretory phospholipase A2 as secreted and also located in the cell membrane and recycling endosome. Tissue expression noted by UniProt (human): Expressed in kidney, heart, liver, and skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
