| Field | Specification |
|---|---|
| Target | |
| Alternative names | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1; PLC-154; Phosphoinositide phospholipase C-beta-1; Phospholipase C-I; PLC-I; Phospholipase C-beta-1; PLC-beta-1; PLCB1; KIAA0581 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PLCB1 recombinant protein (Position: H9-D834). Human PLCB1 shares 98.8% and 98.5% amino acid (aa) sequence identity with mouse and rat PLCB1, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 (gene PLCB1) in human, mouse and rat samples and is validated for Western blot and ELISA.
PLCB1 (PLCβ1) is a phospholipase C that cleaves PI(4,5)P2 into the second messengers IP3 and diacylglycerol after activation by Gαq-coupled receptors. It is abundant in the brain and nucleus, and loss-of-function variants are linked to early infantile epileptic encephalopathy.
| Target | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 (gene PLCB1; UniProt Q9NQ66, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human PLCB1 fragment (His9–Asp834), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 98.8% and 98.5%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 in the nuclear membrane and cytoplasm.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
