| Field | Specification |
|---|---|
| Target | |
| Alternative names | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1; Pancreas-enriched phospholipase C; Phosphoinositide phospholipase C-epsilon-1; Phospholipase C-epsilon-1; PLC-epsilon-1; PLCE1; KIAA1516; PLCE; PPLC |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PLCE1 recombinant protein (Position: E4-E735). Human PLCE1 shares 74.6% and 73.6% amino acid (aa) sequence identity with mouse and rat PLCE1, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (gene PLCE1) in human samples and is validated for Western blot and ELISA.
PLCE1 (PLCε) is a large bifunctional enzyme that both hydrolyzes PI(4,5)P2 and acts as a guanine nucleotide exchange factor for Ras-family GTPases. Activated by Ras, Rap and heterotrimeric G proteins, it links several signaling inputs, and recessive PLCE1 mutations cause early-onset nephrotic syndrome.
| Target | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (gene PLCE1; UniProt Q9P212, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human PLCE1 fragment (Glu4–Glu735), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 74.6% and 73.6%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 in the cytoplasm, cell membrane and Golgi apparatus membrane. Tissue expression noted by UniProt (human): Widely expressed.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
