| Field | Specification |
|---|---|
| Target | |
| Alternative names | Plectin;PCN;PLTN;Hemidesmosomal protein 1;HD1;Plectin-1;PLEC;PLEC1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Plectin |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFE-16 is a rabbit monoclonal antibody that recognizes plectin in human, mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 531.8 kDa, and the supplier reports an observed band at about 531 kDa.
Plectin is a very large cytolinker protein that connects intermediate filaments to actin, microtubules and cell junctions such as hemidesmosomes and desmosomes. It gives mechanical strength to skin, muscle and other tissues, and PLEC mutations cause epidermolysis bullosa simplex with muscular dystrophy and related disorders.
| Target | Plectin (gene PLEC; UniProt Q15149, human) |
|---|---|
| Clone | AFE-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 531.8 kDa |
| Observed MW | 531 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:30 |
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human plectin.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places plectin in the cytoplasm, cell junction and cell projection. Tissue expression noted by UniProt (human): Widely expressed with highest levels in muscle, heart, placenta and spinal cord. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
