| Field | Specification |
|---|---|
| Target | |
| Alternative names | Pleckstrin homology domain-containing family B member 1; PH domain-containing family B member 1; Evectin-1; PH domain-containing protein in retina 1; PHRET1; Pleckstrin homology domain retinal protein 1; PLEKHB1; EVT1; KPL1; PHR1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PLEKHB1 recombinant protein (Position: H82-S214). Human PLEKHB1 shares 93.9% and 92.4% amino acid (aa) sequence identity with mouse and rat PLEKHB1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes pleckstrin homology domain-containing family B member 1 (gene PLEKHB1) in human, monkey, mouse and rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 27.2 kDa, and the supplier reports an observed band at about 27 kDa.
PLEKHB1 (also called evectin-1) is a small pleckstrin homology domain-containing membrane protein. Its function has not been fully characterized.
| Target | Pleckstrin homology domain-containing family B member 1 (gene PLEKHB1; UniProt Q9UF11, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Monkey, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 27.2 kDa |
| Observed MW | 27 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Monkey, Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human SH-SY5Y cells, human HepG2 cells, human U251 cells, monkey RF/6A cells, rat eye tissue, mouse eye tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human PLEKHB1 fragment (His82–Ser214), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 93.9% and 92.4%, respectively.
Reactivity Notes
The supplier lists reactivity with human, monkey, mouse and rat. UniProt places pleckstrin homology domain-containing family B member 1 in the membrane and cytoplasm. Tissue expression noted by UniProt (human): Highly expressed in retina and brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
