| Field | Specification |
|---|---|
| Target | |
| Alternative names | Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1; Lysyl hydroxylase 1; LH1; PLOD1; LLH; PLOD |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PLOD1 recombinant protein (Position: K23-A640). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (gene PLOD1) in human samples and is validated for Western blot and ELISA. The predicted molecular weight is 83.6 kDa, and the supplier reports an observed band at about 84 kDa.
PLOD1 (lysyl hydroxylase 1) is an endoplasmic reticulum enzyme that hydroxylates lysine residues in collagen chains. The resulting hydroxylysines are needed for glycosylation and stable cross-linking of collagen fibrils, and PLOD1 deficiency causes the kyphoscoliotic form of Ehlers-Danlos syndrome.
| Target | Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (gene PLOD1; UniProt Q02809, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 83.6 kDa |
| Observed MW | 84 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human U87 cells, human HepG2 cells, human U251 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human PLOD1 fragment (Lys23–Ala640), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 in the rough endoplasmic reticulum membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
