Anti-PMP70 ABCD3 Monoclonal Antibody

SKU:BHA21009126
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Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-ABCD3 antibody (Rabbit monoclonal, clone AFAF-1, Rabbit IgG). Validated for Western blot, immunofluorescence and flow cytometry. Reactivity: Human, Mouse, Rat. Recognizes ATP-binding cassette sub-family D member 3.
Target ABCD3
clone number AFAF-1
Host Rabbit
Reactivity Human,Mouse,Rat
Isotype Rabbit IgG
Application(s) WB, ICC, IF, Flow
Options selector
Catalog no. Size Conjugation
M02370 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      Form: Liquid
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      Applications: WB,ICC,IF,Flow Cytometry
      Application details: WB 1:500-2000<br>ICC/IF 1:50-200<br>FC 1:100<br>
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target ABCD3
Alternative names ATP-binding cassette sub-family D member 3; 70 kDa peroxisomal membrane protein; PMP70; ABCD3; PXMP1
Gene ID 5825
UniProt # P28288
Host Rabbit
Clone AFAF-1
Clonality
  • Monoclonal
Isotype
  • Rabbit IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
  • Immunofluorescence
  • Flow Cytometry
Immunogen A synthesized peptide derived from human PMP70
Molecular weight 75.5 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Peroxisome membrane; Multi-pass membrane protein.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M02370
Main SKU BHA21009126
Rabbit Monoclonal

Product Overview

Clone AFAF-1 is a rabbit monoclonal antibody that recognizes ATP-binding cassette sub-family D member 3 (gene ABCD3) in human, mouse and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 75.5 kDa, and the supplier reports an observed band at about 70 kDa.

PMP70 (ABCD3) is an abundant ATP-binding cassette transporter of the peroxisomal membrane. It brings branched-chain and long-chain fatty acyl-CoAs and bile acid intermediates into peroxisomes for oxidation, and it is widely used as a marker of peroxisomes.

Target ATP-binding cassette sub-family D member 3 (gene ABCD3; UniProt P28288, human)
Clone AFAF-1
Host / Clonality / Isotype Rabbit / Monoclonal / Rabbit IgG
Reactivity Human, Mouse, Rat
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 75.5 kDa
Observed MW 70 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000
Immunocytochemistry / Immunofluorescence 1:50–1:200
Flow cytometry 1:100

Immunogen

Synthetic peptide based on the sequence of human PMP70.

Reactivity Notes

The supplier lists reactivity with human, mouse and rat. UniProt places ATP-binding cassette sub-family D member 3 in the peroxisome membrane.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000; Immunocytochemistry / Immunofluorescence 1:50–1:200; Flow cytometry 1:100. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse, Rat. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 75.5 kDa; observed band at about 70 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human PMP70.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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