| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitochondrial-processing peptidase subunit alpha; Alpha-MPP; Inactive zinc metalloprotease alpha; P-55; PMPCA; INPP5E; KIAA0123; MPPA |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PMPCA/INPP5 recombinant protein (Position: R99-R525). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects mitochondrial-processing peptidase subunit alpha (gene PMPCA) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 58.3 kDa, and the supplier reports an observed band at about 50–58 kDa.
PMPCA is the alpha subunit of the mitochondrial processing peptidase (MPP), located in the mitochondrial matrix. It recognizes and binds the N-terminal targeting sequences of newly imported precursor proteins, allowing the beta subunit to cut these presequences away. This processing step is required for most matrix proteins to mature, making PMPCA relevant to mitochondrial protein import research.
| Target | Mitochondrial-processing peptidase subunit alpha (gene PMPCA; UniProt Q10713, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 58.3 kDa |
| Observed MW | 50–58 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human MCF-7 cells, human A549 cells, human T-47D cells, rat liver tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue, human lymphoma tissue, human renal cancer tissue, human tonsil tissue, human breast cancer tissue, human gall bladder adenosquamous carcinoma tissue, mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HEP3B cells.
Samples with a confirmed signal (flow cytometry): U937 cells.
Immunogen
Recombinant human PMPCA/INPP5 fragment (Arg99–Arg525), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places mitochondrial-processing peptidase subunit alpha in the mitochondrion matrix and mitochondrion inner membrane. Tissue expression noted by UniProt (human): Ubiquitously expressed with highest expression in fetal tissues and adult brain, cerebellum and cerebellar vermis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
