| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phosphomevalonate kinase; PMKase; hPMK; PMVK; PMKI |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PMVK recombinant protein (Position: K22-L192). Human PMVK shares 82.5% amino acid (aa) sequence identity with mouse PMVK. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects phosphomevalonate kinase (gene PMVK) in human, mouse and rat samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA.
Phosphomevalonate kinase (PMVK) is a cytosolic enzyme of the mevalonate pathway. It uses ATP to convert mevalonate 5-phosphate into mevalonate 5-diphosphate, a step toward isopentenyl diphosphate, the building block for cholesterol and other isoprenoids. It is studied in sterol and isoprenoid metabolism.
| Target | Phosphomevalonate kinase (gene PMVK; UniProt Q15126, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human PMVK fragment (Lys22–Leu192), expressed in E. coli. Sequence identity with the mouse ortholog: 82.5%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places phosphomevalonate kinase in the cytoplasm. Tissue expression noted by UniProt (human): Heart, liver, skeletal muscle, kidney, and pancreas.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
