| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bifunctional polynucleotide phosphatase/kinase; DNA 5'-kinase/3'-phosphatase; Polynucleotide kinase-3'-phosphatase; PNKP |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PNK / PNKP |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 29P36 is a rabbit monoclonal antibody that recognizes bifunctional polynucleotide phosphatase/kinase (gene PNKP) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 57.1 kDa, and the supplier reports an observed band at about 57 kDa.
Polynucleotide kinase 3′-phosphatase (PNKP) is a DNA repair enzyme with two activities: it removes 3′-phosphate groups and adds phosphate to 5′-hydroxyl ends. By cleaning up broken DNA ends so polymerases and ligases can act, it supports base excision repair, single-strand break repair and non-homologous end joining.
| Target | Bifunctional polynucleotide phosphatase/kinase (gene PNKP; UniProt Q96T60, human) |
|---|---|
| Clone | 29P36 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 57.1 kDa |
| Observed MW | 57 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human PNK / PNKP.
Reactivity Notes
The supplier lists reactivity with human. UniProt places bifunctional polynucleotide phosphatase/kinase in the nucleus and chromosome. Tissue expression noted by UniProt (human): Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
