| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phenylethanolamine N-methyltransferase; PNMTase; Noradrenaline N-methyltransferase; PNMT; PENT |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PNMT |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 28P11 is a rabbit monoclonal antibody that recognizes phenylethanolamine N-methyltransferase (gene PNMT) in human and mouse samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 30.9 kDa, and the supplier reports an observed band at about 30 kDa.
Phenylethanolamine N-methyltransferase (PNMT) makes adrenaline (epinephrine) by adding a methyl group from S-adenosylmethionine to noradrenaline. It is highly expressed in the adrenal medulla and in some brainstem neurons, and it is widely used as a marker of adrenaline-producing cells in studies of the stress response and adrenal tumors.
| Target | Phenylethanolamine N-methyltransferase (gene PNMT; UniProt P11086, human) |
|---|---|
| Clone | 28P11 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 30.9 kDa |
| Observed MW | 30 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human PNMT.
Reactivity Notes
The supplier lists reactivity with human and mouse. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
