| Field | Specification |
|---|---|
| Target | |
| Alternative names | Purine nucleoside phosphorylase; PNP; Inosine phosphorylase; Inosine-guanosine phosphorylase; NP |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PNP recombinant protein (Position: Q44-Q273). Human PNP shares 86.5% and 87% amino acid (aa) sequence identity with mouse and rat PNP, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets purine nucleoside phosphorylase (gene PNP) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA.
Purine nucleoside phosphorylase (PNP) is an enzyme of the purine salvage pathway that cleaves inosine and guanosine, and their deoxy forms, into the free base and (deoxy)ribose-1-phosphate. Loss of PNP activity causes toxic accumulation of deoxyguanosine in T cells, which is why PNP deficiency leads to immunodeficiency and why PNP inhibitors have been developed for T-cell cancers.
| Target | Purine nucleoside phosphorylase (gene PNP; UniProt P00491, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human PNP fragment (Gln44–Gln273), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 86.5% and 87%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places purine nucleoside phosphorylase in the cytoplasm. Tissue expression noted by UniProt (human): Expressed in red blood cells.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
