| Field | Specification |
|---|---|
| Target | |
| Alternative names | Omega-hydroxyceramide transacylase; Patatin-like phospholipase domain-containing protein 1; PNPLA1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PNPLA1 recombinant protein (Position: K141-D496). Human PNPLA1 shares 51.5% amino acid (aa) sequence identity with mouse PNPLA1. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects omega-hydroxyceramide transacylase (gene PNPLA1) in human samples and is validated for Western blot and ELISA.
PNPLA1 is an enzyme in the epidermis that transfers linoleic acid from triglycerides onto omega-hydroxyceramides. This produces omega-O-acylceramides, highly hydrophobic lipids needed to build the lipid layers of the stratum corneum and the skin's water barrier. PNPLA1 mutations cause a form of autosomal recessive congenital ichthyosis.
| Target | Omega-hydroxyceramide transacylase (gene PNPLA1; UniProt Q8N8W4, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human PNPLA1 fragment (Lys141–Asp496), expressed in E. coli. Sequence identity with the mouse ortholog: 51.5%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places omega-hydroxyceramide transacylase in the cytoplasm. Tissue expression noted by UniProt (human): Expressed in the digestive system.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
