Anti-PNPT1 Antibody Picoband®

Overview

Anti-PNPT1 Antibody Picoband® is an antibody for PNPT1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: PNPT1 (prion like protein doppel); UniProt: Q8TCS8
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 86 kDa, calculated 39411 MW
• Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-PNPT1 Antibody Picoband® catalog # A05460-1.

Biological background

Biological context: Required for normal acrosome reaction and for normal male fertility (By similarity). Can bind Cu (2+) (PubMed:15218028, PubMed:20411530).

Expression and localization notes: cellular localization: Cell membrane., tissue context: Expressed in testis, in Sertoli cells, ejaculated spermatozoa and in seminal fluid (at protein level)..

Common research applications

• Western blotting (WB): Compare PNPT1 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of PNPT1 in tissue sections, considering fixation and antigen retrieval effects.
• Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
• Flow cytometry: Quantify PNPT1-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: The protein encoded by this gene belongs to the evolutionary conserved polynucleotide phosphorylase family comprised of phosphate dependent 3'-to-5' exoribonucleases implicated in RNA processing and degradation. This enzyme is predominantly localized in the mitochondrial intermembrane space and is involved in import of RNA to mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency-13 and autosomal recessive nonsyndromic deafness-70. Related pseudogenes are found on chromosomes 3 and 7.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cell membrane.
• Tissue details: Expressed in testis, in Sertoli cells, ejaculated spermatozoa and in seminal fluid (at protein level).
• Research category: Neurodegenerative Disease,Neurology Process,Neuroscience

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.