Anti-Podoplanin PDPN Rabbit Monoclonal Antibody

SKU:BHA21008497
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Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-PDPN antibody (Rabbit monoclonal, clone AACC-16, Rabbit IgG). Validated for Western blot. Reactivity: Human, Mouse, Rat. Recognizes podoplanin. Observed band in Western blot: about 36 kDa.
Target PDPN
clone number AACC-16
Host Rabbit
Reactivity Human,Mouse,Rat
Isotype Rabbit IgG
Application(s) WB
Options selector
Catalog no. Size Conjugation
M01124-1 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      Form: Liquid
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      Applications: WB
      Application details: WB 1:500-2000<br>
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target PDPN
Alternative names Podoplanin;Aggrus;Glycoprotein 36;Gp36;PA2.26 antigen;T1-alpha;T1A;PDPN ;GP36 ;PSEC0003, PSEC0025;
Gene ID 10630
UniProt # Q86YL7
Host Rabbit
Clone AACC-16
Clonality
  • Monoclonal
Isotype
  • Rabbit IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
Immunogen A synthesized peptide derived from human Podoplanin
Molecular weight 16.7 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Membrane ; Single-pass type I membrane protein . Cell projection, filopodium membrane ; Single-pass type I membrane protein . Cell projection, lamellipodium membrane ; Single-pass type I membrane protein . Cell projection, microvillus membrane ; Single-pass type I membrane protein . Cell projection, ruffle membrane ; Single-pass type I membrane protein . Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles. .
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M01124-1
Main SKU BHA21008497
Rabbit Monoclonal

Product Overview

Clone AACC-16 is a rabbit monoclonal antibody that recognizes podoplanin (gene PDPN) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 16.7 kDa, and the supplier reports an observed band at about 36 kDa.

Podoplanin (PDPN, also called gp36 or T1α) is a mucin-type transmembrane glycoprotein expressed on lymphatic endothelium, type I lung cells, podocytes and fibroblastic reticular cells. It binds the platelet receptor CLEC-2 to trigger platelet activation, which helps separate lymphatic from blood vessels during development, and it is a standard lymphatic marker also studied in cell migration and cancer.

Target Podoplanin (gene PDPN; UniProt Q86YL7, human)
Clone AACC-16
Host / Clonality / Isotype Rabbit / Monoclonal / Rabbit IgG
Reactivity Human, Mouse, Rat
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 16.7 kDa
Observed MW 36 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000

Immunogen

Synthetic peptide based on the sequence of human podoplanin.

Reactivity Notes

The supplier lists reactivity with human, mouse and rat. UniProt places podoplanin in the [Podoplanin]: Membrane, cell projection, membrane raft and apical cell membrane. Tissue expression noted by UniProt (human): Highly expressed in placenta, lung, skeletal muscle and brain.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse, Rat. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 16.7 kDa; observed band at about 36 kDa. A difference from the calculated size is common and can reflect post-translational modification, processing or isoform differences.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human podoplanin.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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