| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein O-glucosyltransferase 1; CAP10-like 46 kDa protein; hCLP46; KTEL motif-containing protein 1; Myelodysplastic syndromes relative protein; O-glucosyltransferase Rumi homolog; hRumi; Protein O-xylosyltransferase POGLUT1; POGLUT1; C3orf9; CLP46; KTELC1; MDSRP |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at C-terminus of human POGLUT1, which shares 92.6% and 88.9% amino acid (aa) sequence identity with mouse and rat POGLUT1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets protein O-glucosyltransferase 1 (gene POGLUT1) in human and mouse samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 46.2 kDa, and the supplier reports an observed band at about 55 kDa.
Protein O-glucosyltransferase 1 (POGLUT1) adds glucose or xylose to specific serine residues within EGF-like repeats of secreted and membrane proteins. Its best-known target is the Notch receptor, and this modification supports normal Notch signaling and muscle development. POGLUT1 mutations cause a form of limb-girdle muscular dystrophy.
| Target | Protein O-glucosyltransferase 1 (gene POGLUT1; UniProt Q8NBL1, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 46.2 kDa |
| Observed MW | 55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
Samples with a confirmed band (WB): human HL-60 cells, human HepG2 cells, human U87 cells, human Raji cells, human Jurkat cells, human HeLa cells, mouse thymus tissue, mouse Ana-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human breast cancer tissue, human gallbladder adenocarcinoma tissue, human gastric cancer tissue, human lung cancer tissue, human lymphoma tissue, human ovarian cancer tissue, human placenta tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): T-47D cells.
Immunogen
Synthetic peptide based on the sequence of at C-terminus of human POGLUT1. Sequence identity with the mouse and rat orthologs: 92.6% and 88.9%, respectively.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places protein O-glucosyltransferase 1 in the endoplasmic reticulum lumen. Tissue expression noted by UniProt (human): Expressed in most adult tissues at different intensities. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
