| Field | Specification |
|---|---|
| Target | |
| Alternative names | Pogo transposable element with ZNF domain; Suppressor of hairy wing homolog 5; Zinc finger protein 280E; Zinc finger protein 635; POGZ; KIAA0461; SUHW5; ZNF280E; ZNF635 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human POGZ recombinant protein (Position: D23-R1110). Human POGZ shares 93.7% amino acid (aa) sequence identity with mouse POGZ. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects pogo transposable element with ZNF domain (gene POGZ) in human samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA.
POGZ is a zinc-finger nuclear protein with a domain related to pogo transposases. It associates with heterochromatin protein HP1 (CBX5) and contributes to kinetochore assembly, sister chromatid cohesion and proper chromosome segregation in mitosis, and it supports homologous recombination repair. POGZ variants are linked to neurodevelopmental disorders.
| Target | Pogo transposable element with ZNF domain (gene POGZ; UniProt Q7Z3K3, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human POGZ fragment (Asp23–Arg1110), expressed in E. coli. Sequence identity with the mouse ortholog: 93.7%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places pogo transposable element with ZNF domain in the nucleus, chromosome and cytoplasm.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
